Detailed information of TA system TA146203

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 3011521..3012163
• Replicon: chromosome
• Accession: NZ_CP047422
• Organism: Shewanella algae strain 18064-CSB-B-B

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: GM320_RS14345
• Protein ID: WP_144218476.1
• Coordinates: 3011741..3012163 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A7X5Z2N1
• Locus tag: GM320_RS14340
• Protein ID: WP_028779782.1
• Coordinates: 3011521..3011760 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM320_RS14315	3007204..3008031	+	828	WP_071238279.1	prolipoprotein diacylglyceryl transferase	-
GM320_RS14320	3008031..3008825	+	795	WP_071238278.1	thymidylate synthase	-
GM320_RS14325	3008942..3010117	+	1176	WP_025010547.1	Na+/H+ antiporter NhaA	-
GM320_RS14330	3010129..3010497	+	369	WP_096141176.1	hypothetical protein	-
GM320_RS14335	3010514..3011422	+	909	WP_025010548.1	transcriptional activator NhaR	-
GM320_RS14340	3011521..3011760	+	240	WP_028779782.1	succinate dehydrogenase assembly factor 2	Antitoxin
GM320_RS14345	3011741..3012163	+	423	WP_144218476.1	hypothetical protein	Toxin
GM320_RS14350	3012181..3013791	-	1611	WP_025010550.1	L-aspartate oxidase	-
GM320_RS14355	3013991..3014569	+	579	WP_025010551.1	RNA polymerase sigma factor RpoE	-
GM320_RS14360	3014594..3015214	+	621	WP_159352951.1	anti-sigma factor	-
GM320_RS14365	3015224..3016156	+	933	WP_159352952.1	MucB/RseB C-terminal domain-containing protein	-
GM320_RS14370	3016167..3016646	+	480	WP_159352953.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-135)

Antitoxin

• Flavinator of succinate dehydrogenase (5-75)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16286.83 Da
• Isoelectric Point: 7.5565

>T146203 WP_144218476.1 NZ_CP047422:3011741-3012163 [Shewanella algae]
VAGQRHRFQLSSSFSQYLSLTVLAAICLSSFLAWPALDYPFYLPFKCLCLLLMLAGFGLALWRLRRWQLCFWLNELGEGQ
FEPGSSFELSGRPWVSPFVVTFTYQGEDNYGRCWLFADMFDDTDYRHLCRLLLQRSKSAN

Nucleotide

• Length: 423 bp

>T146203 NZ_CP062729:4209122-4209340 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9247.74 Da
• Isoelectric Point: 5.0633

>AT146203 WP_028779782.1 NZ_CP047422:3011521-3011760 [Shewanella algae]
MSTPRVKWACRRGMLELDVLFQPFVEQHYETLSPEDKALFVRLLECEDPELFAWFMGHEQCPDPQLAQMVVRVRGRAAP

Nucleotide

• Length: 240 bp

>AT146203 NZ_CP062729:4208722-4209096 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z2N1