Detailed information of TA system TA146201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 888959..890508
• Replicon: chromosome
• Accession: NZ_CP047422
• Organism: Shewanella algae strain 18064-CSB-B-B

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GM320_RS04735
• Protein ID: WP_159352467.1
• Coordinates: 889207..890508 (+)
• Length: 434 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GM320_RS04730
• Protein ID: WP_025011264.1
• Coordinates: 888959..889207 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM320_RS04715	884425..886350	+	1926	WP_159352465.1	U32 family peptidase	-
GM320_RS04720	886361..886735	+	375	WP_014386412.1	PH domain-containing protein	-
GM320_RS04725	886748..888445	-	1698	WP_159352466.1	ATP-binding cassette domain-containing protein	-
GM320_RS04730	888959..889207	+	249	WP_025011264.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GM320_RS04735	889207..890508	+	1302	WP_159352467.1	type II toxin-antitoxin system HipA family toxin	Toxin
GM320_RS04740	890649..892016	-	1368	WP_028781110.1	L-cystine transporter	-
GM320_RS04745	892320..894347	+	2028	WP_159352468.1	S9 family peptidase	-
GM320_RS04750	894425..895009	-	585	WP_159352469.1	LysE family translocator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 434 a.a.
• Molecular weight: 48616.51 Da
• Isoelectric Point: 9.6959

>T146201 WP_159352467.1 NZ_CP047422:889207-890508 [Shewanella algae]
MQKLIAYMNGELVGTLAKHKNGAHTFQYDKDWITNAMARPLSLSLRLQLPPITSDAVINYFDNLLPDSPQVRDRIVARYK
ASSKQPFDLLKEIGKDSVGAIALFPPEHPYKKEPLSYEILDNKKLEAVLSAYKSDIPLGMLKEEEDFRISVAGAQEKTAL
LFIDGQWCIPKGNTPTTHIIKLPIGEIPQANATLDLKDSVENEYLCIELAKALGFAVPSVNIIKTDNLKALAVERFDRRW
TKDRAGLLRLPQEDICQVFGMPSSIKYESQGGPGIAQIMKLLMGSSNALEDRYNFMRFQVFQWIIGATDGHAKNFSIYID
RGGSYRLTPFYDILSAYPLLGGKGLNIRQLKLAMGLKATKGKKYEINKILPRHFLDTAKAVNFSQDKMQRIMDEMKGALP
KAISQVRANLPEGFPEHISNSIFENAKKIIKKI

Nucleotide

• Length: 1302 bp

>T146201 NZ_CP062729:3577191-3577478 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9366.70 Da
• Isoelectric Point: 6.2564

>AT146201 WP_025011264.1 NZ_CP047422:888959-889207 [Shewanella algae]
MIYSPKQLADMMLLIRQKNGWTQSELAKKVGIKQATISNFENNPNKTTLSTFFKLVQAMDLTLSIQEKTQLTSPSENDDE
SW

Nucleotide

• Length: 249 bp

>AT146201 NZ_CP062729:3577000-3577191 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA