Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 124099..124700 Replicon plasmid pMS6193A-NDM
Accession NZ_CP047406
Organism Escherichia coli strain MS6193

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag MS6193_RS24570 Protein ID WP_001216034.1
Coordinates 124099..124479 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MS6193_RS24575 Protein ID WP_001190712.1
Coordinates 124479..124700 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6193_RS24535 119150..120082 - 933 WP_000081352.1 homocysteine S-methyltransferase -
MS6193_RS24540 120069..121472 - 1404 WP_001373486.1 S-methylmethionine permease -
MS6193_RS24545 121680..122696 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
MS6193_RS24550 122924..123241 + 318 WP_001513659.1 hypothetical protein -
MS6193_RS24555 123191..123604 - 414 Protein_155 DDE-type integrase/transposase/recombinase -
MS6193_RS24560 123528..123887 - 360 WP_001513660.1 hypothetical protein -
MS6193_RS24565 123915..124094 - 180 WP_001513661.1 hypothetical protein -
MS6193_RS24570 124099..124479 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MS6193_RS24575 124479..124700 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MS6193_RS24580 124883..126439 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
MS6193_RS24585 126436..127719 + 1284 WP_001553855.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaNDM-1 / sul1 / qacE / aadA2 / dfrA12 - 1..142890 142890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T146193 WP_001216034.1 NZ_CP047406:c124479-124099 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T146193 NZ_CP062729:2314406-2314509 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT146193 WP_001190712.1 NZ_CP047406:c124700-124479 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT146193 NZ_CP062729:c2314547-2314276 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References