Detailed information of TA system TA146185

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4596823..4597658
• Replicon: chromosome
• Accession: NZ_CP047405
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: MS6193_RS22130
• Protein ID: WP_001094445.1
• Coordinates: 4596823..4597200 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MS6193_RS22135
• Protein ID: WP_024174331.1
• Coordinates: 4597290..4597658 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS22110	4592915..4594453	-	1539	WP_001187170.1	lysine decarboxylation/transport transcriptional activator CadC	-
MS6193_RS22115	4595202..4596044	-	843	WP_001280499.1	DUF4942 domain-containing protein	-
MS6193_RS22120	4596129..4596326	-	198	WP_000838536.1	DUF957 domain-containing protein	-
MS6193_RS22125	4596338..4596826	-	489	WP_000761717.1	hypothetical protein	-
MS6193_RS22130	4596823..4597200	-	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
MS6193_RS22135	4597290..4597658	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MS6193_RS22140	4597732..4597953	-	222	WP_000692346.1	DUF987 domain-containing protein	-
MS6193_RS22145	4598022..4598498	-	477	WP_001186725.1	RadC family protein	-
MS6193_RS22150	4598514..4598999	-	486	WP_000214415.1	antirestriction protein	-
MS6193_RS22155	4599091..4599423	-	333	Protein_4314	DUF932 domain-containing protein	-
MS6193_RS22160	4599426..4600206	-	781	Protein_4315	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4585426..4616181	30755
	-	flank	IS/Tn	-	-	4599928..4600206	278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T146185 WP_001094445.1 NZ_CP047405:c4597200-4596823 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T146185 NZ_CP062729:365365-365640 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT146185 WP_024174331.1 NZ_CP047405:c4597658-4597290 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT146185 NZ_CP062729:365637-365996 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6