Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3823094..3823725 Replicon chromosome
Accession NZ_CP047405
Organism Escherichia coli strain MS6193

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag MS6193_RS18680 Protein ID WP_001259388.1
Coordinates 3823450..3823725 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MS6193_RS18675 Protein ID WP_000593555.1
Coordinates 3823094..3823453 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6193_RS18650 3819226..3820170 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
MS6193_RS18655 3820167..3821000 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
MS6193_RS18660 3821000..3821764 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
MS6193_RS18665 3821761..3822567 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
MS6193_RS18670 3822573..3822974 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
MS6193_RS18675 3823094..3823453 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MS6193_RS18680 3823450..3823725 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
MS6193_RS18685 3823798..3824922 - 1125 WP_001314210.1 ABC transporter permease -
MS6193_RS18690 3824922..3827657 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
MS6193_RS18695 3827654..3828721 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T146182 WP_001259388.1 NZ_CP047405:c3823725-3823450 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T146182 NZ_CP062728:51522-51827 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT146182 WP_000593555.1 NZ_CP047405:c3823453-3823094 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT146182 NZ_CP062728:51302-51520 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References