Detailed information of TA system TA146178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2795227..2795852
• Replicon: chromosome
• Accession: NZ_CP047405
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MS6193_RS13645
• Protein ID: WP_000911330.1
• Coordinates: 2795227..2795625 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: MS6193_RS13650
• Protein ID: WP_000450524.1
• Coordinates: 2795625..2795852 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS13625	2791134..2791334	+	201	WP_000383836.1	YpfN family protein	-
MS6193_RS13630	2791415..2792113	-	699	WP_000679812.1	esterase	-
MS6193_RS13635	2792187..2794202	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
MS6193_RS13640	2794217..2795080	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
MS6193_RS13645	2795227..2795625	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MS6193_RS13650	2795625..2795852	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MS6193_RS13655	2796006..2796719	-	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
MS6193_RS13660	2796932..2797966	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
MS6193_RS13665	2797983..2798861	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MS6193_RS13670	2799007..2799579	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
MS6193_RS13675	2799579..2800049	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T146178 WP_000911330.1 NZ_CP047405:c2795625-2795227 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T146178 NZ_CP062727:4822219-4822560 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT146178 WP_000450524.1 NZ_CP047405:c2795852-2795625 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT146178 NZ_CP062727:c4822224-4822148 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A829CM33