Detailed information of TA system TA146176

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2261952..2262787
• Replicon: chromosome
• Accession: NZ_CP047405
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: MS6193_RS11090
• Protein ID: WP_000854752.1
• Coordinates: 2262410..2262787 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: MS6193_RS11085
• Protein ID: WP_001278232.1
• Coordinates: 2261952..2262320 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS11045	2257694..2258044	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
MS6193_RS11050	2258041..2258466	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
MS6193_RS11055	2258788..2259243	+	456	WP_000581504.1	hypothetical protein	-
MS6193_RS11060	2259322..2259555	+	234	WP_001119729.1	DUF905 family protein	-
MS6193_RS11065	2259655..2260473	+	819	WP_001234620.1	DUF945 domain-containing protein	-
MS6193_RS11070	2260528..2261013	+	486	WP_000849582.1	antirestriction protein	-
MS6193_RS11075	2261029..2261505	+	477	WP_001347688.1	RadC family protein	-
MS6193_RS11080	2261568..2261789	+	222	WP_000692329.1	DUF987 domain-containing protein	-
MS6193_RS11085	2261952..2262320	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
MS6193_RS11090	2262410..2262787	+	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
MS6193_RS11095	2262784..2262987	+	204	WP_000761705.1	hypothetical protein	-
MS6193_RS11100	2263000..2263113	+	114	WP_001161660.1	DUF957 domain-containing protein	-
MS6193_RS11105	2263272..2264300	+	1029	Protein_2173	IS110 family transposase	-
MS6193_RS11110	2264754..2264936	+	183	WP_024167529.1	ethanolamine utilization protein	-
MS6193_RS11115	2265037..2265366	-	330	WP_000450409.1	DUF496 family protein	-
MS6193_RS11120	2265538..2266596	-	1059	WP_074499974.1	FUSC family protein	-
MS6193_RS11125	2266795..2267268	-	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T146176 WP_000854752.1 NZ_CP047405:2262410-2262787 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T146176 NZ_CP062727:4474646-4474906 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT146176 WP_001278232.1 NZ_CP047405:2261952-2262320 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT146176 NZ_CP062727:4474367-4474627 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72