Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1599907..1600545 Replicon chromosome
Accession NZ_CP047405
Organism Escherichia coli strain MS6193

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag MS6193_RS07710 Protein ID WP_000813794.1
Coordinates 1599907..1600083 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag MS6193_RS07715 Protein ID WP_001270286.1
Coordinates 1600129..1600545 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6193_RS07690 1595526..1596740 - 1215 WP_001348055.1 BenE family transporter YdcO -
MS6193_RS07695 1596793..1597329 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
MS6193_RS07700 1597402..1599363 + 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
MS6193_RS07705 1599455..1599685 - 231 WP_000494244.1 YncJ family protein -
MS6193_RS07710 1599907..1600083 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
MS6193_RS07715 1600129..1600545 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
MS6193_RS07720 1600624..1602030 + 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
MS6193_RS07725 1602275..1603420 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
MS6193_RS07730 1603438..1604451 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
MS6193_RS07735 1604452..1605393 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1594338..1595486 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T146166 WP_000813794.1 NZ_CP047405:1599907-1600083 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T146166 NZ_CP062727:2642218-2642373 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT146166 WP_001270286.1 NZ_CP047405:1600129-1600545 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT146166 NZ_CP062727:c2642206-2642148 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References