Detailed information of TA system TA146154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 547997..548834
• Replicon: chromosome
• Accession: NZ_CP047405
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: MS6193_RS02625
• Protein ID: WP_000227784.1
• Coordinates: 547997..548539 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MS6193_RS02630
• Protein ID: WP_001297137.1
• Coordinates: 548523..548834 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS02605	543536..544447	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
MS6193_RS02610	544615..545106	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
MS6193_RS02615	545234..546598	-	1365	WP_001000974.1	MFS transporter	-
MS6193_RS02620	547006..547941	+	936	WP_001365761.1	sel1 repeat family protein	-
MS6193_RS02625	547997..548539	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
MS6193_RS02630	548523..548834	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MS6193_RS02635	549019..549909	-	891	WP_000971336.1	heme o synthase	-
MS6193_RS02640	549921..550250	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MS6193_RS02645	550250..550864	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MS6193_RS02650	550854..552845	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MS6193_RS02655	552867..553814	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T146154 WP_000227784.1 NZ_CP047405:c548539-547997 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T146154 NZ_CP062726:71588-71746 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT146154 WP_001297137.1 NZ_CP047405:c548834-548523 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT146154 NZ_CP062726:71325-71523 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3