Detailed information of TA system TA146150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 286101..286731
• Replicon: chromosome
• Accession: NZ_CP047393
• Organism: Pontibacillus sp. HMF3514

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0A5HW17
• Locus tag: GS400_RS01645
• Protein ID: WP_027446721.1
• Coordinates: 286381..286731 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GS400_RS01640
• Protein ID: WP_197063073.1
• Coordinates: 286101..286376 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS400_RS01620	281532..281888	+	357	WP_160098510.1	holo-ACP synthase	-
GS400_RS01625	282024..283532	+	1509	WP_160098512.1	NAD(P)H-hydrate dehydratase	-
GS400_RS01630	283581..284600	+	1020	WP_160098514.1	outer membrane lipoprotein carrier protein LolA	-
GS400_RS01635	284766..285926	+	1161	WP_201450152.1	alanine racemase	-
GS400_RS01640	286101..286376	+	276	WP_197063073.1	antitoxin	Antitoxin
GS400_RS01645	286381..286731	+	351	WP_027446721.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GS400_RS01650	286992..287861	+	870	WP_160098518.1	RsbT co-antagonist protein RsbRA	-
GS400_RS01655	287858..288223	+	366	WP_197063072.1	STAS domain-containing protein	-
GS400_RS01660	288227..288628	+	402	WP_027446724.1	anti-sigma regulatory factor	-
GS400_RS01665	288642..289652	+	1011	WP_160098520.1	PP2C family protein-serine/threonine phosphatase	-
GS400_RS01670	289720..290055	+	336	WP_160098522.1	STAS domain-containing protein	-
GS400_RS01675	290055..290534	+	480	WP_027446727.1	anti-sigma B factor RsbW	-
GS400_RS01680	290506..291294	+	789	WP_160098524.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12927.91 Da
• Isoelectric Point: 4.6579

>T146150 WP_027446721.1 NZ_CP047393:286381-286731 [Pontibacillus sp. HMF3514]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAERYGFERDSVILLEQI
RTIDKQRLTDKITNLDDSMMTKVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T146150 NZ_CP062725:c5034754-5034404 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10669.02 Da
• Isoelectric Point: 4.5202

>AT146150 WP_197063073.1 NZ_CP047393:286101-286376 [Pontibacillus sp. HMF3514]
VSENSQEIKIKLPQNLLNELDGMVEEDNVDRNDFIYQATKMYLRERKKRHIRESMREGYMEMAKINLNIASEAFQAEEEA
ETTCERLVSGV

Nucleotide

• Length: 276 bp

>AT146150 NZ_CP062725:c5034999-5034748 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A5HW17