Detailed information of TA system TA146135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1563659..1564212
• Replicon: chromosome
• Accession: NZ_CP047391
• Organism: Serratia marcescens strain 1602

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A2V4FZ31
• Locus tag: GRX59_RS07425
• Protein ID: WP_019454426.1
• Coordinates: 1563898..1564212 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: GRX59_RS07420
• Protein ID: WP_039566839.1
• Coordinates: 1563659..1563895 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRX59_RS07405	1560126..1561661	+	1536	WP_033646792.1	glutathione ABC transporter substrate-binding protein GsiB	-
GRX59_RS07410	1561714..1562634	+	921	WP_033646790.1	glutathione ABC transporter permease GsiC	-
GRX59_RS07415	1562644..1563552	+	909	WP_049233469.1	glutathione ABC transporter permease GsiD	-
GRX59_RS07420	1563659..1563895	+	237	WP_039566839.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GRX59_RS07425	1563898..1564212	+	315	WP_019454426.1	CcdB family protein	Toxin
GRX59_RS07430	1564252..1565094	-	843	WP_015377214.1	S-formylglutathione hydrolase	-
GRX59_RS07435	1565109..1566233	-	1125	WP_033646788.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
GRX59_RS07440	1566264..1567184	-	921	WP_016928453.1	LysR family transcriptional regulator	-
GRX59_RS07445	1567290..1568447	+	1158	WP_033646786.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11745.68 Da
• Isoelectric Point: 5.0488

>T146135 WP_019454426.1 NZ_CP047391:1563898-1564212 [Serratia marcescens]
MQFTVYANRGNSAVYPLLLDVTSDIIGQLNRRVVIPLLPVEKYPGSTRPERLIPLIRLIDDNEYAVMTYEMASIPVRALG
AEFCDVSQYRTRIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T146135 NZ_CP062725:1093265-1093600 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9068.10 Da
• Isoelectric Point: 5.7070

>AT146135 WP_039566839.1 NZ_CP047391:1563659-1563895 [Serratia marcescens]
MPVKQRTTQSVTMTVERSLLSRAREAGINLSATLTAALDAELRQHERIKWQEENREALEALNRFNDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT146135 NZ_CP062725:1093017-1093265 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2V4FZ31