Detailed information of TA system TA146132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 14633..15234
• Replicon: chromosome
• Accession: NZ_CP047391
• Organism: Serratia marcescens strain 1602

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GRX59_RS00070
• Protein ID: WP_074026019.1
• Coordinates: 14633..15013 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S4X5L5
• Locus tag: GRX59_RS00075
• Protein ID: WP_039568270.1
• Coordinates: 15013..15234 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRX59_RS00045	9886..11166	+	1281	WP_047569945.1	DUF3748 domain-containing protein	-
GRX59_RS00050	11197..11544	-	348	WP_033636538.1	YceK/YidQ family lipoprotein	-
GRX59_RS00055	11855..12268	+	414	WP_004933919.1	heat shock chaperone IbpA	-
GRX59_RS00060	12369..12797	+	429	WP_004933922.1	heat shock chaperone IbpB	-
GRX59_RS00065	12964..14622	+	1659	WP_033649549.1	putative transporter	-
GRX59_RS00070	14633..15013	-	381	WP_074026019.1	type II toxin-antitoxin system VapC family toxin	Toxin
GRX59_RS00075	15013..15234	-	222	WP_039568270.1	ribbon-helix-helix protein, CopG family	Antitoxin
GRX59_RS00080	15310..16560	-	1251	WP_060388266.1	valine--pyruvate transaminase	-
GRX59_RS00085	16696..18759	-	2064	WP_060438299.1	alpha-amylase	-
GRX59_RS24335	18956..19108	+	153	WP_019455508.1	hypothetical protein	-
GRX59_RS00090	19110..20087	-	978	WP_016929588.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14201.53 Da
• Isoelectric Point: 7.3170

>T146132 WP_074026019.1 NZ_CP047391:c15013-14633 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIVWTTAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T146132 NZ_CP062725:c708872-708408 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8607.55 Da
• Isoelectric Point: 4.4791

>AT146132 WP_039568270.1 NZ_CP047391:c15234-15013 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHIPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT146132 NZ_CP062725:c709207-708872 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S4X5L5