Detailed information of TA system TA146131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 643733..644358
• Replicon: chromosome
• Accession: NZ_CP047387
• Organism: Agrobacterium sp. CGMCC 11546

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q7D0B1
• Locus tag: GSF67_RS03280
• Protein ID: WP_006311244.1
• Coordinates: 643999..644358 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q7D0B2
• Locus tag: GSF67_RS03275
• Protein ID: WP_010971270.1
• Coordinates: 643733..643999 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSF67_RS03250	638758..639582	+	825	WP_035214768.1	class D beta-lactamase	-
GSF67_RS03255	639622..640230	+	609	WP_006311234.1	class I SAM-dependent methyltransferase	-
GSF67_RS03260	640406..642991	+	2586	WP_006311240.1	heavy metal translocating P-type ATPase	-
GSF67_RS03265	642988..643410	+	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
GSF67_RS03270	643463..643663	+	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
GSF67_RS03275	643733..643999	+	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GSF67_RS03280	643999..644358	+	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GSF67_RS03285	644366..644614	-	249	WP_006311245.1	hypothetical protein	-
GSF67_RS03290	644793..646181	+	1389	WP_006311246.1	MFS transporter	-
GSF67_RS03295	646261..646434	-	174	WP_006311253.1	hypothetical protein	-
GSF67_RS03300	646503..648221	-	1719	WP_006311254.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12857.04 Da
• Isoelectric Point: 8.2782

>T146131 WP_006311244.1 NZ_CP047387:643999-644358 [Agrobacterium sp. CGMCC 11546]
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Nucleotide

• Length: 360 bp

>T146131 NZ_CP062725:c457211-456726 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9423.02 Da
• Isoelectric Point: 5.1106

>AT146131 WP_010971270.1 NZ_CP047387:643733-643999 [Agrobacterium sp. CGMCC 11546]
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL

Nucleotide

• Length: 267 bp

>AT146131 NZ_CP062725:c457480-457202 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: Q7D0B1

Antitoxin

• AlphaFold DB: Q7D0B2