Detailed information of TA system TA146128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 5296561..5297168
• Replicon: chromosome
• Accession: NZ_CP047386
• Organism: Pandoraea fibrosis strain 7641

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8T8BTD3
• Locus tag: PJ20_RS23360
• Protein ID: WP_052240851.1
• Coordinates: 5296561..5296869 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5E4W9D0
• Locus tag: PJ20_RS23365
• Protein ID: WP_039373022.1
• Coordinates: 5296866..5297168 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PJ20_RS23350	5294555..5295847	+	1293	WP_039373028.1	serine hydrolase	-
PJ20_RS23355	5295819..5296163	-	345	WP_039373025.1	GIY-YIG nuclease family protein	-
PJ20_RS23360	5296561..5296869	+	309	WP_052240851.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PJ20_RS23365	5296866..5297168	+	303	WP_039373022.1	putative addiction module antidote protein	Antitoxin
PJ20_RS23370	5297260..5298729	+	1470	WP_039373021.1	SLC13 family permease	-
PJ20_RS23375	5298925..5300058	+	1134	WP_080759322.1	methyl-accepting chemotaxis protein	-
PJ20_RS23380	5300094..5300522	-	429	WP_039373020.1	hypothetical protein	-
PJ20_RS23385	5300527..5301201	-	675	WP_039373018.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (7-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11434.19 Da
• Isoelectric Point: 10.4496

>T146128 WP_052240851.1 NZ_CP047386:5296561-5296869 [Pandoraea fibrosis]
MYVINQSRAFRTWLKGLRDTAAKARILVRIKRAEMGNFGDTKSLGAGLWEMRIDVGAGYRVYYGQDEALTYLLVCGGDKS
TQSADIARAKIVWANVKKESSR

Nucleotide

• Length: 309 bp

>T146128 NZ_CP062725:365365-365640 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10741.14 Da
• Isoelectric Point: 4.9916

>AT146128 WP_039373022.1 NZ_CP047386:5296866-5297168 [Pandoraea fibrosis]
MSHPDITRFDASEYLDNDDMIAEYLNAALDENDAATFLSAVADVAKAKGMTQVALAAGLGRESLYKALAPNAQPRFFTIL
RVMHALGVTFSAVPNRTAHG

Nucleotide

• Length: 303 bp

>AT146128 NZ_CP062725:365637-365996 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5E4W9D0