Detailed information of TA system TA146124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 5296562..5297169
• Replicon: chromosome
• Accession: NZ_CP047385
• Organism: Pandoraea fibrosis strain 6399

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8T8BTD3
• Locus tag: PI93_RS23365
• Protein ID: WP_052240851.1
• Coordinates: 5296562..5296870 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5E4W9D0
• Locus tag: PI93_RS23370
• Protein ID: WP_039373022.1
• Coordinates: 5296867..5297169 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PI93_RS23355	5294556..5295848	+	1293	WP_039373028.1	serine hydrolase	-
PI93_RS23360	5295820..5296164	-	345	WP_039373025.1	GIY-YIG nuclease family protein	-
PI93_RS23365	5296562..5296870	+	309	WP_052240851.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PI93_RS23370	5296867..5297169	+	303	WP_039373022.1	putative addiction module antidote protein	Antitoxin
PI93_RS23375	5297261..5298730	+	1470	WP_039373021.1	SLC13 family permease	-
PI93_RS23380	5298926..5300059	+	1134	WP_080759322.1	methyl-accepting chemotaxis protein	-
PI93_RS23385	5300095..5300523	-	429	WP_039373020.1	hypothetical protein	-
PI93_RS23390	5300528..5301202	-	675	WP_039373018.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (7-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11434.19 Da
• Isoelectric Point: 10.4496

>T146124 WP_052240851.1 NZ_CP047385:5296562-5296870 [Pandoraea fibrosis]
MYVINQSRAFRTWLKGLRDTAAKARILVRIKRAEMGNFGDTKSLGAGLWEMRIDVGAGYRVYYGQDEALTYLLVCGGDKS
TQSADIARAKIVWANVKKESSR

Nucleotide

• Length: 309 bp

>T146124 NZ_CP062724:2065-2340 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10741.14 Da
• Isoelectric Point: 4.9916

>AT146124 WP_039373022.1 NZ_CP047385:5296867-5297169 [Pandoraea fibrosis]
MSHPDITRFDASEYLDNDDMIAEYLNAALDENDAATFLSAVADVAKAKGMTQVALAAGLGRESLYKALAPNAQPRFFTIL
RVMHALGVTFSAVPNRTAHG

Nucleotide

• Length: 303 bp

>AT146124 NZ_CP062724:1781-2065 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E4W9D0