Detailed information of TA system TA146122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5030691..5031256
• Replicon: chromosome
• Accession: NZ_CP047385
• Organism: Pandoraea fibrosis strain 6399

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8T8BJU7
• Locus tag: PI93_RS22170
• Protein ID: WP_039370059.1
• Coordinates: 5030885..5031256 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A5E4VA17
• Locus tag: PI93_RS22165
• Protein ID: WP_010804388.1
• Coordinates: 5030691..5030888 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PI93_RS22140	5026424..5027545	+	1122	WP_052240646.1	ABC transporter substrate-binding protein	-
PI93_RS22145	5027553..5028437	+	885	WP_039370050.1	NAD(P)-dependent oxidoreductase	-
PI93_RS22155	5029027..5029734	-	708	WP_039370052.1	phospholipase D family protein	-
PI93_RS22160	5030086..5030394	-	309	WP_039370056.1	hypothetical protein	-
PI93_RS22165	5030691..5030888	+	198	WP_010804388.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
PI93_RS22170	5030885..5031256	+	372	WP_039370059.1	type II toxin-antitoxin system VapC family toxin	Toxin
PI93_RS22175	5031620..5032702	-	1083	WP_039370062.1	NAD(P)/FAD-dependent oxidoreductase	-
PI93_RS22180	5032699..5033595	-	897	WP_080759167.1	helix-turn-helix transcriptional regulator	-
PI93_RS22185	5033713..5035041	+	1329	WP_052240634.1	FAD-dependent monooxygenase	-
PI93_RS22190	5035102..5035965	+	864	WP_039370065.1	NAD(P)H-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13362.56 Da
• Isoelectric Point: 6.8800

>T146122 WP_039370059.1 NZ_CP047385:5030885-5031256 [Pandoraea fibrosis]
MILVDTSVWIDHLRAGDATLVQLLETGRVLAHPYVIGELALGSLKNRDVVISTLRDLPRVAVATDDEVLRFIGEQTLFGL
GIGYVDAHLLAATRLTPGAAIWTRDKRLAATAERLSLAAKILH

Nucleotide

• Length: 372 bp

>T146122 NZ_CP062723:c4962668-4962318 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7177.21 Da
• Isoelectric Point: 6.3084

>AT146122 WP_010804388.1 NZ_CP047385:5030691-5030888 [Pandoraea fibrosis]
MRTTLALDDELLAKAQAFTGLQEKSALIREALKALIERESARRLARLGGTEADLSDIPRRQTEPA

Nucleotide

• Length: 198 bp

>AT146122 NZ_CP062723:c4962913-4962662 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E4VA17