Detailed information of TA system TA146113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 155720..156245
• Replicon: plasmid pCAU16175_2
• Accession: NZ_CP047380
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: GTP13_RS30425
• Protein ID: WP_001159868.1
• Coordinates: 155720..156025 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: GTP13_RS30430
• Protein ID: WP_000813634.1
• Coordinates: 156027..156245 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS30405	151497..152468	-	972	WP_000817031.1	ParB/RepB/Spo0J family plasmid partition protein	-
GTP13_RS30410	152468..153634	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
GTP13_RS30415	154222..154866	-	645	WP_096245693.1	replication initiation protein RepE	-
GTP13_RS30420	154913..155719	-	807	WP_096245694.1	site-specific integrase	-
GTP13_RS30425	155720..156025	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
GTP13_RS30430	156027..156245	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GTP13_RS30435	156704..157387	+	684	WP_010891291.1	DNA-binding protein	-
GTP13_RS30440	157384..158004	+	621	WP_096317676.1	hypothetical protein	-
GTP13_RS30445	158001..158789	+	789	WP_033561991.1	DUF2971 domain-containing protein	-
GTP13_RS30450	158825..159565	+	741	WP_000933099.1	abortive infection family protein	-
GTP13_RS30455	159724..160389	-	666	WP_053265868.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / toxB / toxB / espC / espT	1..161176	161176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T146113 WP_001159868.1 NZ_CP047380:c156025-155720 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T146113 NZ_CP062723:c3372284-3371910 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT146113 WP_000813634.1 NZ_CP047380:c156245-156027 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT146113 NZ_CP062723:c3372705-3372331 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2