Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 85433..87056 Replicon plasmid pCAU16175_1
Accession NZ_CP047379
Organism Escherichia coli strain CAU16175

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag GTP13_RS28920 Protein ID WP_000088046.1
Coordinates 85731..87056 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag GTP13_RS28915 Protein ID WP_024136327.1
Coordinates 85433..85726 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTP13_RS28890 81305..82546 + 1242 WP_001053338.1 VWA domain-containing protein -
GTP13_RS28895 83109..83390 - 282 WP_000077926.1 hypothetical protein -
GTP13_RS28900 83440..83631 - 192 WP_000398480.1 hypothetical protein -
GTP13_RS28905 83723..84064 - 342 WP_001151575.1 hypothetical protein -
GTP13_RS28910 84437..84829 + 393 WP_000341066.1 hypothetical protein -
GTP13_RS28915 85433..85726 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
GTP13_RS28920 85731..87056 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
GTP13_RS28925 87117..87323 + 207 WP_000134171.1 hypothetical protein -
GTP13_RS28930 87425..87835 + 411 WP_000985911.1 hypothetical protein -
GTP13_RS28935 87848..88663 + 816 WP_001572439.1 HNH endonuclease -
GTP13_RS28940 88917..89342 + 426 WP_001043843.1 hypothetical protein -
GTP13_RS28945 89796..90033 + 238 Protein_86 hypothetical protein -
GTP13_RS28950 90088..90387 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR / sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / tet(A) - 1..190219 190219


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T146111 WP_000088046.1 NZ_CP047379:85731-87056 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T146111 NZ_CP062723:3114084-3114191 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT146111 WP_024136327.1 NZ_CP047379:85433-85726 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT146111 NZ_CP062723:c3114036-3113977 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References