Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4608212..4608866 Replicon chromosome
Accession NZ_CP047378
Organism Escherichia coli strain CAU16175

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GTP13_RS23645 Protein ID WP_000244777.1
Coordinates 4608212..4608619 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GTP13_RS23650 Protein ID WP_000354046.1
Coordinates 4608600..4608866 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTP13_RS23625 4604169..4605902 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
GTP13_RS23630 4605908..4606618 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GTP13_RS23635 4606643..4607539 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GTP13_RS23640 4607651..4608172 + 522 WP_001055874.1 flavodoxin FldB -
GTP13_RS23645 4608212..4608619 - 408 WP_000244777.1 toxin CptA Toxin
GTP13_RS23650 4608600..4608866 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GTP13_RS23655 4609109..4610089 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
GTP13_RS23660 4610285..4610944 - 660 WP_000250274.1 hemolysin III family protein -
GTP13_RS23665 4611108..4611419 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GTP13_RS23670 4611464..4612897 + 1434 WP_001341859.1 6-phospho-beta-glucosidase BglA -
GTP13_RS23675 4612954..4613697 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T146107 WP_000244777.1 NZ_CP047378:c4608619-4608212 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T146107 NZ_CP062723:2317986-2318089 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT146107 WP_000354046.1 NZ_CP047378:c4608866-4608600 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT146107 NZ_CP062723:c2318127-2317856 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References