Detailed information of TA system TA146104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4116107..4116732
• Replicon: chromosome
• Accession: NZ_CP047378
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GTP13_RS21205
• Protein ID: WP_000911330.1
• Coordinates: 4116107..4116505 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GTP13_RS21210
• Protein ID: WP_000450524.1
• Coordinates: 4116505..4116732 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS21185	4111985..4112185	+	201	WP_000383836.1	YpfN family protein	-
GTP13_RS21190	4112295..4112993	-	699	WP_000679812.1	esterase	-
GTP13_RS21195	4113067..4115082	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
GTP13_RS21200	4115097..4115960	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
GTP13_RS21205	4116107..4116505	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GTP13_RS21210	4116505..4116732	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GTP13_RS21215	4116886..4117599	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GTP13_RS21220	4117812..4118846	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GTP13_RS21225	4118863..4119741	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GTP13_RS21230	4119887..4120459	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GTP13_RS21235	4120459..4120929	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T146104 WP_000911330.1 NZ_CP047378:c4116505-4116107 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T146104 NZ_CP062723:751347-751661 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT146104 WP_000450524.1 NZ_CP047378:c4116732-4116505 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT146104 NZ_CP062723:751658-752074 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33