Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1365940..1366558 Replicon chromosome
Accession NZ_CP047378
Organism Escherichia coli strain CAU16175

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GTP13_RS06620 Protein ID WP_001291435.1
Coordinates 1365940..1366158 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GTP13_RS06625 Protein ID WP_000344800.1
Coordinates 1366184..1366558 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTP13_RS06585 1361230..1361802 + 573 WP_000779826.1 lipoprotein -
GTP13_RS06590 1361833..1362144 - 312 WP_000409911.1 MGMT family protein -
GTP13_RS06600 1362522..1362875 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GTP13_RS06605 1362917..1364467 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GTP13_RS06610 1364631..1365101 - 471 WP_000136192.1 YlaC family protein -
GTP13_RS06615 1365217..1365768 - 552 WP_096245422.1 maltose O-acetyltransferase -
GTP13_RS06620 1365940..1366158 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GTP13_RS06625 1366184..1366558 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GTP13_RS06630 1367104..1370253 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
GTP13_RS06635 1370276..1371469 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146079 WP_001291435.1 NZ_CP047378:c1366158-1365940 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T146079 NZ_CP062721:c2594374-2594096 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT146079 WP_000344800.1 NZ_CP047378:c1366558-1366184 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT146079 NZ_CP062721:c2594567-2594376 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References