Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2545677..2546317 Replicon chromosome
Accession NZ_CP047376
Organism Aeromonas salmonicida subsp. salmonicida strain J410

Toxin (Protein)


Gene name vagD Uniprot ID A0A807Z4U5
Locus tag GO994_RS12350 Protein ID WP_005320982.1
Coordinates 2545677..2546090 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A807Z5T0
Locus tag GO994_RS12355 Protein ID WP_005320985.1
Coordinates 2546087..2546317 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO994_RS12320 2541660..2541899 - 240 WP_005311129.1 hypothetical protein -
GO994_RS12325 2542506..2543143 + 638 Protein_2355 M20/M25/M40 family metallo-hydrolase -
GO994_RS12330 2543381..2544298 + 918 WP_088814108.1 IS5-like element ISAs4 family transposase -
GO994_RS12335 2544389..2544697 - 309 WP_017413136.1 hypothetical protein -
GO994_RS12340 2544719..2545219 - 501 WP_005320977.1 hypothetical protein -
GO994_RS12345 2545246..2545599 - 354 WP_017413138.1 hypothetical protein -
GO994_RS12350 2545677..2546090 - 414 WP_005320982.1 PIN domain-containing protein Toxin
GO994_RS12355 2546087..2546317 - 231 WP_005320985.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GO994_RS12360 2546386..2546772 - 387 WP_017413139.1 tyrosine-type recombinase/integrase -
GO994_RS12365 2546685..2547074 - 390 WP_159368364.1 hypothetical protein -
GO994_RS12370 2547491..2548408 - 918 WP_076611341.1 IS5-like element ISAs4 family transposase -
GO994_RS12375 2548498..2549730 - 1233 Protein_2365 carbohydrate-binding protein -
GO994_RS12380 2550066..2551295 - 1230 WP_005311315.1 methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 2543381..2548408 5027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-42)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.7146

>T146069 WP_005320982.1 NZ_CP047376:c2546090-2545677 [Aeromonas salmonicida subsp. salmonicida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Download         Length: 414 bp

>T146069 NZ_CP062721:283377-283484 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8699.88 Da        Isoelectric Point: 4.7419

>AT146069 WP_005320985.1 NZ_CP047376:c2546317-2546087 [Aeromonas salmonicida subsp. salmonicida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Download         Length: 231 bp

>AT146069 NZ_CP062721:c283329-283263 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807Z4U5

Antitoxin

Source ID Structure
AlphaFold DB A0A807Z5T0

References