Detailed information of TA system TA146068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 60827..61383
• Replicon: plasmid p1AsJ409
• Accession: NZ_CP047375
• Organism: Aeromonas salmonicida subsp. salmonicida strain J409

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GO992_RS22440
• Protein ID: WP_159365015.1
• Coordinates: 60827..61102 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GO992_RS22445
• Protein ID: WP_174416604.1
• Coordinates: 61102..61383 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO992_RS22400	56144..56506	-	363	WP_139394881.1	type II toxin-antitoxin system PemK/MazF family toxin	-
GO992_RS22405	56506..56766	-	261	WP_139394880.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GO992_RS22410	56867..57252	-	386	Protein_77	hypothetical protein	-
GO992_RS22415	57449..57544	+	96	Protein_78	IS5/IS1182 family transposase	-
GO992_RS22420	57541..58503	-	963	Protein_79	IS630 family transposase	-
GO992_RS22425	58592..58921	-	330	WP_159365013.1	YcaO-like family protein	-
GO992_RS22430	58918..59124	-	207	WP_159365014.1	hypothetical protein	-
GO992_RS22435	59126..60040	-	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
GO992_RS22440	60827..61102	-	276	WP_159365015.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GO992_RS22445	61102..61383	-	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
GO992_RS22450	61595..62155	+	561	WP_042468955.1	recombinase family protein	-
GO992_RS22455	62159..65187	+	3029	Protein_86	Tn3 family transposase	-
GO992_RS22460	65222..65896	+	675	Protein_87	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ati2 / ati1 / ascL / ascK / ascJ / ascI / ascH / ascG / ascF / ascE / ascD / ascC / ascB / exsD / exsA / exsB / exsE / exsC / aopD / aopB / acrH / acrV / acrG / acrR / ascV / ascY / ascX / acr2 / acr1 / aopN / ascN / ascO / ascP / ascQ / ascR / ascS / ascT / ascU / sycO / aopO / ati2 / ati1 / ati2 / ati2 / ati1 / ascL / ascK / ascJ	1..99881	99881
	-	inside	IScluster/Tn	-	sycO / aopO / ati2 / ati1 / ati2 / ati2 / ati1	42477..97584	55107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10642.40 Da
• Isoelectric Point: 8.4730

>T146068 WP_159365015.1 NZ_CP047375:c61102-60827 [Aeromonas salmonicida subsp. salmonicida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLLLWHTRENR

Nucleotide

• Length: 276 bp

>T146068 NZ_CP062720:71588-71746 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT146068 WP_174416604.1 NZ_CP047375:c61383-61102 [Aeromonas salmonicida subsp. salmonicida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT146068 NZ_CP062720:71325-71523 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC