Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 765853..766493 Replicon chromosome
Accession NZ_CP047374
Organism Aeromonas salmonicida subsp. salmonicida strain J409

Toxin (Protein)


Gene name vagD Uniprot ID A0A807Z4U5
Locus tag GO992_RS03515 Protein ID WP_005320982.1
Coordinates 765853..766266 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A807Z5T0
Locus tag GO992_RS03520 Protein ID WP_005320985.1
Coordinates 766263..766493 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO992_RS03490 762682..763319 + 638 Protein_676 M20/M25/M40 family metallo-hydrolase -
GO992_RS03495 763557..764474 + 918 WP_088814108.1 IS5-like element ISAs4 family transposase -
GO992_RS03500 764565..764873 - 309 WP_017413136.1 hypothetical protein -
GO992_RS03505 764895..765395 - 501 WP_005320977.1 hypothetical protein -
GO992_RS03510 765422..765775 - 354 WP_017413138.1 hypothetical protein -
GO992_RS03515 765853..766266 - 414 WP_005320982.1 PIN domain-containing protein Toxin
GO992_RS03520 766263..766493 - 231 WP_005320985.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GO992_RS03525 766562..766948 - 387 WP_017413139.1 tyrosine-type recombinase/integrase -
GO992_RS03530 766861..767250 - 390 WP_159368364.1 hypothetical protein -
GO992_RS03535 767667..768584 - 918 WP_076611341.1 IS5-like element ISAs4 family transposase -
GO992_RS03540 768674..769906 - 1233 Protein_686 carbohydrate-binding protein -
GO992_RS03545 770242..771471 - 1230 WP_005311315.1 methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 763557..768584 5027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-42)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.7146

>T146067 WP_005320982.1 NZ_CP047374:c766266-765853 [Aeromonas salmonicida subsp. salmonicida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Download         Length: 414 bp

>T146067 NZ_CP062720:51520-51825 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8699.88 Da        Isoelectric Point: 4.7419

>AT146067 WP_005320985.1 NZ_CP047374:c766493-766263 [Aeromonas salmonicida subsp. salmonicida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Download         Length: 231 bp

>AT146067 NZ_CP062720:51300-51518 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807Z4U5

Antitoxin

Source ID Structure
AlphaFold DB A0A807Z5T0

References