Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 236290..236932 Replicon chromosome
Accession NZ_CP047366
Organism Bacillus nitratireducens strain BM02

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag GSN03_RS01290 Protein ID WP_000635965.1
Coordinates 236582..236932 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag GSN03_RS01285 Protein ID WP_000004570.1
Coordinates 236290..236577 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSN03_RS01260 (GSN03_01260) 231604..232566 + 963 WP_000961171.1 UV DNA damage repair endonuclease UvsE -
GSN03_RS01265 (GSN03_01265) 232559..233131 - 573 WP_212088842.1 rhomboid family intramembrane serine protease -
GSN03_RS01270 (GSN03_01270) 233224..233583 + 360 WP_000635042.1 holo-ACP synthase -
GSN03_RS01275 (GSN03_01275) 233740..234690 + 951 WP_002100392.1 outer membrane lipoprotein carrier protein LolA -
GSN03_RS01280 (GSN03_01280) 234809..235978 + 1170 WP_212088844.1 alanine racemase -
GSN03_RS01285 (GSN03_01285) 236290..236577 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
GSN03_RS01290 (GSN03_01290) 236582..236932 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GSN03_RS01295 (GSN03_01295) 237001..239169 + 2169 WP_212091465.1 Tex family protein -
GSN03_RS01300 (GSN03_01300) 239227..239343 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
GSN03_RS01305 (GSN03_01305) 239554..240015 + 462 WP_044739196.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T146065 WP_000635965.1 NZ_CP047366:236582-236932 [Bacillus nitratireducens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T146065 NZ_CP062719:c5098379-5098206 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT146065 WP_000004570.1 NZ_CP047366:236290-236577 [Bacillus nitratireducens]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT146065 NZ_CP062719:c5098703-5098407 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References