Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2103779..2104270 Replicon chromosome
Accession NZ_CP047363
Organism Macrococcus canis strain Epi0076A

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag GTN30_RS11230 Protein ID WP_164953882.1
Coordinates 2103779..2104129 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GTN30_RS11235 Protein ID WP_138072445.1
Coordinates 2104130..2104270 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTN30_RS11210 2101099..2101866 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
GTN30_RS11215 2101871..2102314 - 444 WP_138072439.1 anti-sigma B factor RsbW -
GTN30_RS11220 2102319..2102642 - 324 WP_086043353.1 anti-sigma factor antagonist -
GTN30_RS11225 2102723..2103730 - 1008 WP_138072441.1 PP2C family protein-serine/threonine phosphatase -
GTN30_RS11230 2103779..2104129 - 351 WP_164953882.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GTN30_RS11235 2104130..2104270 - 141 WP_138072445.1 antitoxin MazE Antitoxin
GTN30_RS11240 2104332..2105474 - 1143 WP_164953883.1 alanine racemase -
GTN30_RS11245 2105500..2105856 - 357 WP_164953884.1 holo-ACP synthase -
GTN30_RS11250 2105798..2106319 - 522 WP_164953885.1 PH domain-containing protein -
GTN30_RS11255 2106303..2107814 - 1512 WP_164953886.1 PH domain-containing protein -
GTN30_RS11260 2107804..2108280 - 477 WP_138072455.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12735.86 Da        Isoelectric Point: 9.5976

>T146064 WP_164953882.1 NZ_CP047363:c2104129-2103779 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDEALLISLGLSIDSE

Download         Length: 351 bp

>T146064 NZ_CP062719:c5001565-5001215 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT146064 WP_138072445.1 NZ_CP047363:c2104270-2104130 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTIDGE

Download         Length: 141 bp

>AT146064 NZ_CP062719:c5001810-5001559 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References