Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2017929..2018420 Replicon chromosome
Accession NZ_CP047361
Organism Macrococcus canis strain SD607

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag GTN31_RS10575 Protein ID WP_086043355.1
Coordinates 2017929..2018279 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GTN31_RS10580 Protein ID WP_138072445.1
Coordinates 2018280..2018420 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTN31_RS10555 2015249..2016016 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
GTN31_RS10560 2016021..2016464 - 444 WP_086043352.1 anti-sigma B factor RsbW -
GTN31_RS10565 2016469..2016792 - 324 WP_086043353.1 anti-sigma factor antagonist -
GTN31_RS10570 2016873..2017880 - 1008 WP_174804797.1 PP2C family protein-serine/threonine phosphatase -
GTN31_RS10575 2017929..2018279 - 351 WP_086043355.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GTN31_RS10580 2018280..2018420 - 141 WP_138072445.1 antitoxin MazE Antitoxin
GTN31_RS10585 2018482..2019624 - 1143 WP_164942786.1 alanine racemase -
GTN31_RS10590 2019650..2020006 - 357 WP_164942787.1 holo-ACP synthase -
GTN31_RS10595 2019948..2020469 - 522 WP_164942788.1 PH domain-containing protein -
GTN31_RS10600 2020453..2021964 - 1512 WP_164942789.1 PH domain-containing protein -
GTN31_RS10605 2021954..2022430 - 477 WP_086043360.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12705.88 Da        Isoelectric Point: 9.9423

>T146060 WP_086043355.1 NZ_CP047361:c2018279-2017929 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T146060 NZ_CP062719:c4513472-4513074 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT146060 WP_138072445.1 NZ_CP047361:c2018420-2018280 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTIDGE

Download         Length: 141 bp

>AT146060 NZ_CP062719:c4513768-4513475 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References