Detailed information of TA system TA146057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5102829..5104510
• Replicon: chromosome
• Accession: NZ_CP047360
• Organism: Klebsiella variicola strain AHKv-S01

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GSY73_RS25030
• Protein ID: WP_022065798.1
• Coordinates: 5102829..5103746 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A0B7GCX3
• Locus tag: GSY73_RS25035
• Protein ID: WP_008807257.1
• Coordinates: 5103752..5104510 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSY73_RS25010	5098135..5099655	+	1521	WP_008807253.1	sugar ABC transporter ATP-binding protein	-
GSY73_RS25015	5099652..5100650	+	999	WP_004206363.1	ribose ABC transporter permease	-
GSY73_RS25020	5100681..5101622	+	942	WP_117133573.1	ABC transporter substrate-binding protein	-
GSY73_RS25025	5101697..5102704	+	1008	WP_022065799.1	carbohydrate kinase family protein	-
GSY73_RS25030	5102829..5103746	-	918	WP_022065798.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GSY73_RS25035	5103752..5104510	-	759	WP_008807257.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GSY73_RS25040	5104595..5105323	-	729	WP_008807258.1	response regulator transcription factor	-
GSY73_RS25045	5105596..5107743	+	2148	WP_167341479.1	formate dehydrogenase subunit alpha	-
GSY73_RS25050	5108185..5108871	+	687	WP_008807260.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34751.14 Da
• Isoelectric Point: 5.4707

>T146057 WP_022065798.1 NZ_CP047360:c5103746-5102829 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGMNAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T146057 NZ_CP062719:3633570-3633857 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28653.01 Da
• Isoelectric Point: 10.1988

>AT146057 WP_008807257.1 NZ_CP047360:c5104510-5103752 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVTLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT146057 NZ_CP062719:3633379-3633570 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B7GCX3