Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4232993..4233612 Replicon chromosome
Accession NZ_CP047360
Organism Klebsiella variicola strain AHKv-S01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GSY73_RS20860 Protein ID WP_002892050.1
Coordinates 4233394..4233612 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag GSY73_RS20855 Protein ID WP_008805436.1
Coordinates 4232993..4233367 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSY73_RS20845 4228148..4229341 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GSY73_RS20850 4229364..4232510 + 3147 WP_167341413.1 multidrug efflux RND transporter permease subunit AcrB -
GSY73_RS20855 4232993..4233367 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
GSY73_RS20860 4233394..4233612 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GSY73_RS20865 4233771..4234337 + 567 WP_008805435.1 maltose O-acetyltransferase -
GSY73_RS20870 4234309..4234437 - 129 Protein_4049 hypothetical protein -
GSY73_RS20875 4234474..4234944 + 471 WP_008805434.1 YlaC family protein -
GSY73_RS20880 4234913..4236370 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
GSY73_RS20885 4236471..4237169 + 699 WP_060876185.1 GNAT family N-acetyltransferase -
GSY73_RS20890 4237166..4237306 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GSY73_RS20895 4237306..4237569 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T146056 WP_002892050.1 NZ_CP047360:4233394-4233612 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T146056 NZ_CP062719:c3626624-3626469 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT146056 WP_008805436.1 NZ_CP047360:4232993-4233367 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT146056 NZ_CP062719:3626636-3626694 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References