Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2683571..2685159 Replicon chromosome
Accession NZ_CP047360
Organism Klebsiella variicola strain AHKv-S01

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag GSY73_RS13220 Protein ID WP_008804919.1
Coordinates 2683571..2684893 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MCN9
Locus tag GSY73_RS13225 Protein ID WP_008804920.1
Coordinates 2684893..2685159 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSY73_RS13205 2678636..2681137 + 2502 WP_167341290.1 fimbrial biogenesis outer membrane usher protein -
GSY73_RS13210 2681125..2682129 + 1005 WP_008804917.1 type 1 fimbrial protein -
GSY73_RS13215 2682262..2683554 + 1293 WP_016161010.1 glycoside hydrolase family 10 protein -
GSY73_RS13220 2683571..2684893 - 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
GSY73_RS13225 2684893..2685159 - 267 WP_008804920.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
GSY73_RS13230 2685425..2685751 - 327 WP_008804921.1 hydroxyisourate hydrolase -
GSY73_RS13235 2685748..2686248 - 501 WP_049139320.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
GSY73_RS13240 2686226..2687605 - 1380 WP_167341291.1 purine permease -
GSY73_RS13245 2687951..2689105 + 1155 WP_023322603.1 FAD-dependent urate hydroxylase HpxO -
GSY73_RS13250 2689086..2690009 - 924 WP_008804925.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T146054 WP_008804919.1 NZ_CP047360:c2684893-2683571 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T146054 NZ_CP062719:c3226468-3226313 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9936.30 Da        Isoelectric Point: 8.5912

>AT146054 WP_008804920.1 NZ_CP047360:c2685159-2684893 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT146054 NZ_CP062719:3226480-3226538 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MCN9

References