Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 279147..279778 Replicon chromosome
Accession NZ_CP047360
Organism Klebsiella variicola strain AHKv-S01

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H4S6Z2
Locus tag GSY73_RS01270 Protein ID WP_008806943.1
Coordinates 279147..279422 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7H4S6Z1
Locus tag GSY73_RS01275 Protein ID WP_023298308.1
Coordinates 279419..279778 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSY73_RS01250 274383..274718 + 336 WP_002921203.1 universal stress protein UspB -
GSY73_RS01255 274778..276274 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
GSY73_RS01260 276504..277697 + 1194 WP_131024432.1 NAD(P)/FAD-dependent oxidoreductase -
GSY73_RS01265 277737..278750 - 1014 WP_012540371.1 magnesium transporter -
GSY73_RS01270 279147..279422 + 276 WP_008806943.1 type II toxin-antitoxin system HicA family toxin Toxin
GSY73_RS01275 279419..279778 + 360 WP_023298308.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GSY73_RS01280 279806..280207 - 402 WP_048268290.1 nickel-responsive transcriptional regulator NikR -
GSY73_RS01285 280195..280986 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
GSY73_RS01290 280983..281747 - 765 WP_023340796.1 nickel import ATP-binding protein NikD -
GSY73_RS01295 281747..282580 - 834 WP_032755951.1 nickel ABC transporter permease subunit NikC -
GSY73_RS01300 282577..283521 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10295.87 Da        Isoelectric Point: 10.3900

>T146042 WP_008806943.1 NZ_CP047360:279147-279422 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVRP

Download         Length: 276 bp

>T146042 NZ_CP062719:408066-408593 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13341.08 Da        Isoelectric Point: 4.4605

>AT146042 WP_023298308.1 NZ_CP047360:279419-279778 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNVFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT146042 NZ_CP062719:407793-408059 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4S6Z2

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4S6Z1

References