Detailed information of TA system TA146038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3930767..3932338
• Replicon: chromosome
• Accession: NZ_CP047352
• Organism: Proteus mirabilis strain ZA25

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B4F0T8
• Locus tag: GTH26_RS18250
• Protein ID: WP_012368637.1
• Coordinates: 3930767..3932080 (-)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: GTH26_RS18255
• Protein ID: WP_004249771.1
• Coordinates: 3932081..3932338 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH26_RS18230	3925937..3926740	+	804	WP_060554978.1	energy transducer TonB	-
GTH26_RS18235	3926794..3928755	-	1962	WP_172767808.1	hypothetical protein	-
GTH26_RS18240	3928787..3929716	-	930	WP_060554980.1	metallophosphoesterase	-
GTH26_RS18245	3929901..3930365	-	465	WP_004246516.1	DUF1097 domain-containing protein	-
GTH26_RS18250	3930767..3932080	-	1314	WP_012368637.1	type II toxin-antitoxin system HipA family toxin	Toxin
GTH26_RS18255	3932081..3932338	-	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
GTH26_RS18260	3932487..3933851	-	1365	WP_004246513.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
GTH26_RS18265	3933989..3935629	-	1641	WP_004246512.1	membrane protein insertase YidC	-
GTH26_RS18270	3935632..3935892	-	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
GTH26_RS18275	3935856..3936215	-	360	WP_004246510.1	ribonuclease P protein component	-
GTH26_RS18280	3936233..3936376	-	144	WP_004246509.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49670.78 Da
• Isoelectric Point: 6.5023

>T146038 WP_012368637.1 NZ_CP047352:c3932080-3930767 [Proteus mirabilis]
MASLDVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPADIKKIEYQKLNDIELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T146038 NZ_CP062718:53972-54277 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT146038 WP_004249771.1 NZ_CP047352:c3932338-3932081 [Proteus mirabilis]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT146038 NZ_CP062718:53752-53970 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: B4F0T8

Antitoxin

• AlphaFold DB: B4F0T9