Detailed information of TA system TA146037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3578317..3579016
• Replicon: chromosome
• Accession: NZ_CP047352
• Organism: Proteus mirabilis strain ZA25

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: GTH26_RS16625
• Protein ID: WP_004249093.1
• Coordinates: 3578317..3578703 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: GTH26_RS16630
• Protein ID: WP_004246828.1
• Coordinates: 3578696..3579016 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH26_RS16610	3574312..3574893	-	582	WP_012368524.1	DNA-3-methyladenine glycosylase I	-
GTH26_RS16615	3575144..3576049	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
GTH26_RS16620	3576059..3578131	+	2073	WP_036919263.1	glycine--tRNA ligase subunit beta	-
GTH26_RS16625	3578317..3578703	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH26_RS16630	3578696..3579016	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
GTH26_RS16635	3579065..3579499	-	435	WP_004246827.1	hypothetical protein	-
GTH26_RS16640	3579492..3580376	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
GTH26_RS16645	3580593..3581879	-	1287	WP_036919265.1	DUF3748 domain-containing protein	-
GTH26_RS16650	3582016..3582633	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
GTH26_RS16655	3582935..3583558	+	624	WP_004246821.1	guanylate kinase	-
GTH26_RS16660	3583613..3583888	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T146037 WP_004249093.1 NZ_CP047352:3578317-3578703 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T146037 NZ_CP062718:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT146037 WP_004246828.1 NZ_CP047352:3578696-3579016 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT146037 NZ_CP062718:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97