Detailed information of TA system TA146035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1720193..1720992
• Replicon: chromosome
• Accession: NZ_CP047352
• Organism: Proteus mirabilis strain ZA25

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: GTH26_RS08220
• Protein ID: WP_004247966.1
• Coordinates: 1720468..1720992 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: GTH26_RS08215
• Protein ID: WP_004247964.1
• Coordinates: 1720193..1720471 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH26_RS08190	1715543..1716625	+	1083	WP_004242680.1	peptide chain release factor 1	-
GTH26_RS08195	1716625..1717473	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
GTH26_RS08200	1717451..1718266	+	816	WP_004242688.1	invasion regulator SirB1	-
GTH26_RS08205	1718324..1719178	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
GTH26_RS08210	1719186..1720115	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
GTH26_RS08215	1720193..1720471	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
GTH26_RS08220	1720468..1720992	+	525	WP_004247966.1	hypothetical protein	Toxin
GTH26_RS08225	1721073..1722773	-	1701	WP_004247967.1	C4-dicarboxylic acid transporter DauA	-
GTH26_RS08235	1723726..1724667	+	942	WP_036918236.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T146035 WP_004247966.1 NZ_CP047352:1720468-1720992 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T146035 NZ_CP062717:c4988180-4987830 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT146035 WP_004247964.1 NZ_CP047352:1720193-1720471 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT146035 NZ_CP062717:c4988425-4988174 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: B4EVS2