Detailed information of TA system TA146027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3287749..3289471
• Replicon: chromosome
• Accession: NZ_CP047349
• Organism: Proteus terrae subsp. cibarius strain ZN2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A6G6SX12
• Locus tag: GTH25_RS15110
• Protein ID: WP_054103802.1
• Coordinates: 3287749..3288687 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A0M0HVB9
• Locus tag: GTH25_RS15115
• Protein ID: WP_002026815.1
• Coordinates: 3288680..3289471 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH25_RS15100	3283903..3284724	-	822	WP_087529689.1	TnsA endonuclease N-terminal domain-containing protein	-
GTH25_RS15105	3284859..3287168	-	2310	Protein_2915	AAA family ATPase	-
GTH25_RS15110	3287749..3288687	-	939	WP_054103802.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GTH25_RS15115	3288680..3289471	-	792	WP_002026815.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GTH25_RS15120	3289692..3290078	-	387	WP_164530709.1	hypothetical protein	-
GTH25_RS15125	3290075..3290647	-	573	WP_164530710.1	type IV conjugative transfer system lipoprotein TraV	-
GTH25_RS15130	3290722..3292011	-	1290	WP_164530711.1	TraB/VirB10 family protein	-
GTH25_RS15135	3292014..3292910	-	897	WP_164530816.1	type-F conjugative transfer system secretin TraK	-
GTH25_RS15140	3292894..3293520	-	627	WP_025441422.1	pilus assembly protein	-
GTH25_RS15145	3293517..3293798	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
GTH25_RS15150	3293979..3294302	+	324	WP_164530712.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(H) / dfrA1	-	3170091..3311563	141472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-97)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35629.99 Da
• Isoelectric Point: 5.0082

>T146027 WP_054103802.1 NZ_CP047349:c3288687-3287749 [Proteus terrae subsp. cibarius]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINIQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHRAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T146027 NZ_CP062717:c3613223-3613068 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30116.50 Da
• Isoelectric Point: 9.1901

>AT146027 WP_002026815.1 NZ_CP047349:c3289471-3288680 [Proteus terrae subsp. cibarius]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLCSGVYYRPDAQGDIKPTWVDAIQALDVQLGVSVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT146027 NZ_CP062717:3613235-3613293 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A6G6SX12

Antitoxin

• AlphaFold DB: A0A0M0HVB9