Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 33997..34574 Replicon plasmid pZN3-cfr-121kb
Accession NZ_CP047346
Organism Proteus vulgaris strain ZN3

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag GTH24_RS21415 Protein ID WP_023159957.1
Coordinates 33997..34329 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GTH24_RS21420 Protein ID WP_096864992.1
Coordinates 34329..34574 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH24_RS21390 30008..30667 - 660 WP_125894568.1 DUF4365 domain-containing protein -
GTH24_RS21395 30836..31651 - 816 WP_164526964.1 site-specific integrase -
GTH24_RS22050 31737..31877 + 141 WP_206535566.1 hypothetical protein -
GTH24_RS21400 32195..32992 + 798 WP_048607643.1 hypothetical protein -
GTH24_RS21405 33357..33500 + 144 WP_071547955.1 type I toxin-antitoxin system Hok family toxin -
GTH24_RS21410 33586..33966 - 381 WP_096864991.1 hypothetical protein -
GTH24_RS21415 33997..34329 - 333 WP_023159957.1 endoribonuclease MazF Toxin
GTH24_RS21420 34329..34574 - 246 WP_096864992.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GTH24_RS21425 34844..35467 + 624 WP_109910889.1 AAA family ATPase -
GTH24_RS21430 35568..35795 + 228 WP_096864993.1 DNA partition complex ParG -
GTH24_RS21435 35893..36261 - 369 WP_109910890.1 hypothetical protein -
GTH24_RS21440 36611..37003 - 393 WP_109910891.1 hypothetical protein -
GTH24_RS21445 37013..37435 - 423 WP_048821775.1 hypothetical protein -
GTH24_RS21450 37428..37772 - 345 WP_023159966.1 single-stranded DNA-binding protein -
GTH24_RS21455 37775..38821 - 1047 WP_072070749.1 ParB/RepB/Spo0J family partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / msr(E) / mph(E) / sul1 / qacE / lnu(F) / aadA2 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / cfr / dfrA12 - 1..121294 121294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T146025 WP_023159957.1 NZ_CP047346:c34329-33997 [Proteus vulgaris]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T146025 NZ_CP062717:3152632-3152739 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8800.23 Da        Isoelectric Point: 5.0508

>AT146025 WP_096864992.1 NZ_CP047346:c34574-34329 [Proteus vulgaris]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT146025 NZ_CP062717:c3152579-3152518 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure

References