Detailed information of TA system TA146018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 2534434..2535173
• Replicon: chromosome
• Accession: NZ_CP047344
• Organism: Proteus vulgaris strain ZN3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A6G6SNF5
• Locus tag: GTH24_RS12065
• Protein ID: WP_072068519.1
• Coordinates: 2534688..2535173 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A6G6SIM7
• Locus tag: GTH24_RS12060
• Protein ID: WP_072068518.1
• Coordinates: 2534434..2534700 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH24_RS12025	2530098..2530580	+	483	WP_006533151.1	flagellar basal body-associated protein FliL	-
GTH24_RS12030	2530586..2531614	+	1029	WP_072068513.1	flagellar motor switch protein FliM	-
GTH24_RS12035	2531607..2532017	+	411	WP_006533153.1	flagellar motor switch protein FliN	-
GTH24_RS12040	2532021..2532467	+	447	WP_072068514.1	flagellar biosynthetic protein FliO	-
GTH24_RS12045	2532467..2533237	+	771	WP_072068515.1	flagellar type III secretion system pore protein FliP	-
GTH24_RS12050	2533253..2533522	+	270	WP_072068516.1	flagellar biosynthesis protein FliQ	-
GTH24_RS12055	2533528..2534310	+	783	WP_164526460.1	flagellar type III secretion system protein FliR	-
GTH24_RS12060	2534434..2534700	+	267	WP_072068518.1	DUF1778 domain-containing protein	Antitoxin
GTH24_RS12065	2534688..2535173	+	486	WP_072068519.1	GNAT family N-acetyltransferase	Toxin
GTH24_RS12070	2535268..2536212	-	945	WP_072068520.1	flagellar hook-associated protein FlgL	-
GTH24_RS12075	2536244..2537896	-	1653	WP_072068521.1	flagellar hook-associated protein FlgK	-
GTH24_RS12080	2538014..2539000	-	987	WP_072068522.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
GTH24_RS12085	2539000..2540106	-	1107	WP_072068523.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-81)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17951.68 Da
• Isoelectric Point: 9.9332

>T146018 WP_072068519.1 NZ_CP047344:2534688-2535173 [Proteus vulgaris]
MGKITAPEPLSNSHEVADFYSSENVLDNWIKQRGLKNQFLGASRTFMVCEKNKKRVVGYYSLATGSVNHTEAISHIRHNM
PDPIPVIILARLAVDKTFHGKGLGADLLRDAVLRCHHVAENIGVRAIMVHALTENAKQFYLHNGFKASSTQENTLFLALK
K

Nucleotide

• Length: 486 bp

>T146018 NZ_CP062717:946470-946877 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10039.56 Da
• Isoelectric Point: 4.6437

>AT146018 WP_072068518.1 NZ_CP047344:2534434-2534700 [Proteus vulgaris]
MKADVQLNIRAKGSQRALIDTAAEILHKSRTDFILETACQAAEDVILDRRTFNLNDAQYAEFIEIIDAPVDIDPALEKLL
MRKPLWEK

Nucleotide

• Length: 267 bp

>AT146018 NZ_CP062717:946223-946489 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6G6SNF5

Antitoxin

• AlphaFold DB: A0A6G6SIM7