Detailed information of TA system TA146015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 12132..12640
• Replicon: plasmid pZF1-cfr
• Accession: NZ_CP047341
• Organism: Proteus terrae subsp. cibarius strain ZF1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GTH23_RS20030
• Protein ID: WP_050878415.1
• Coordinates: 12365..12640 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GTH23_RS20025
• Protein ID: WP_050878414.1
• Coordinates: 12132..12356 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH23_RS19990	7633..7842	-	210	WP_050878402.1	hypothetical protein	-
GTH23_RS19995	7846..8130	-	285	WP_050878404.1	hypothetical protein	-
GTH23_RS20000	8184..8963	-	780	WP_050878406.1	hypothetical protein	-
GTH23_RS20005	9000..9242	-	243	WP_156734530.1	hypothetical protein	-
GTH23_RS20010	9320..9655	-	336	WP_050878410.1	hypothetical protein	-
GTH23_RS20015	10219..10599	-	381	WP_050878412.1	hypothetical protein	-
GTH23_RS20020	10697..11920	-	1224	WP_159364217.1	DUF3560 domain-containing protein	-
GTH23_RS20025	12132..12356	+	225	WP_050878414.1	hypothetical protein	Antitoxin
GTH23_RS20030	12365..12640	+	276	WP_050878415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH23_RS20035	13344..13517	-	174	WP_154393749.1	hypothetical protein	-
GTH23_RS20040	14187..15017	+	831	WP_050878417.1	replication initiation protein	-
GTH23_RS20045	15027..15854	+	828	WP_050878418.1	GIY-YIG nuclease family protein	-
GTH23_RS20050	15951..16286	+	336	WP_050878420.1	hypothetical protein	-
GTH23_RS20055	16394..16687	+	294	WP_050878422.1	sigma-70 family RNA polymerase sigma factor	-
GTH23_RS20060	16880..17188	+	309	WP_050878424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cfr	-	1..59168	59168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (5-70)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11101.99 Da
• Isoelectric Point: 10.1375

>T146015 WP_050878415.1 NZ_CP047341:12365-12640 [Proteus terrae subsp. cibarius]
MDHYRIEYTKRFQKEIKKIDKYHQKIIQNYIEKHIDNSINPRLIGKILKGDKAEFIRYRIGDYRLIATIEDDKLLILALS
IGHRKDIYKHF

Nucleotide

• Length: 276 bp

>T146015 NZ_CP062717:c457209-456724 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8490.46 Da
• Isoelectric Point: 4.2696

>AT146015 WP_050878414.1 NZ_CP047341:12132-12356 [Proteus terrae subsp. cibarius]
MRKNSTKAIRLNDDELTLFESYANAKGLTFSELCKSAVIEKIEDEIDLKLAEESYNEYLNDNETLSHKALFDSL

Nucleotide

• Length: 225 bp

>AT146015 NZ_CP062717:c457478-457200 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA