Detailed information of TA system TA146009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 1029029..1029774
• Replicon: chromosome
• Accession: NZ_CP047340
• Organism: Proteus terrae subsp. cibarius strain ZF1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GTH23_RS04575
• Protein ID: WP_075673025.1
• Coordinates: 1029029..1029520 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: GTH23_RS04580
• Protein ID: WP_075673024.1
• Coordinates: 1029508..1029774 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH23_RS04555	1024114..1025220	+	1107	WP_088495221.1	flagellar basal body P-ring protein FlgI	-
GTH23_RS04560	1025220..1026206	+	987	WP_088495222.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
GTH23_RS04565	1026324..1027967	+	1644	WP_156733630.1	flagellar hook-associated protein FlgK	-
GTH23_RS04570	1027999..1028943	+	945	WP_156733628.1	flagellar hook-associated protein FlgL	-
GTH23_RS04575	1029029..1029520	-	492	WP_075673025.1	GNAT family N-acetyltransferase	Toxin
GTH23_RS04580	1029508..1029774	-	267	WP_075673024.1	DUF1778 domain-containing protein	Antitoxin
GTH23_RS04585	1029899..1030681	-	783	WP_088495227.1	flagellar type III secretion system protein FliR	-
GTH23_RS04590	1030687..1030956	-	270	WP_069367481.1	flagellar biosynthesis protein FliQ	-
GTH23_RS04595	1030972..1031685	-	714	WP_171455369.1	flagellar type III secretion system pore protein FliP	-
GTH23_RS04600	1031742..1032188	-	447	WP_023581829.1	flagellar biosynthetic protein FliO	-
GTH23_RS04605	1032192..1032602	-	411	WP_023581830.1	flagellar motor switch protein FliN	-
GTH23_RS04610	1032595..1033623	-	1029	WP_088495228.1	flagellar motor switch protein FliM	-
GTH23_RS04615	1033629..1034111	-	483	WP_006533151.1	flagellar basal body-associated protein FliL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18228.15 Da
• Isoelectric Point: 10.3194

>T146009 WP_075673025.1 NZ_CP047340:c1029520-1029029 [Proteus terrae subsp. cibarius]
MGKVTAPEPLSNSHEVADFYSSENVLDNWIKQRGLKNQFLGASRTFVVCEKNKKRVVGYYSLATGSVNHTEAMSHIRRNM
PDPIPVIILARLAVDKTFHGKGLGADLLRDAVLRCHHVAENIGVRAIMVHALTENAKHFYLHHGFKASSTQDKILFLALK
KKI

Nucleotide

• Length: 492 bp

>T146009 NZ_CP062716:53971-54276 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10111.62 Da
• Isoelectric Point: 4.5256

>AT146009 WP_075673024.1 NZ_CP047340:c1029774-1029508 [Proteus terrae subsp. cibarius]
MKADVQLNIRAKESQRALIDTAAEILHKSRTDFILETACQAAEDVILDRRTFNLNDAQYAEFIEIIDAPVDIDPALEKLL
MRKPLWEK

Nucleotide

• Length: 267 bp

>AT146009 NZ_CP062716:53751-53969 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA