Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 31273..31916 Replicon plasmid p2019036D-50kb
Accession NZ_CP047338
Organism Klebsiella pneumoniae strain 2019036D

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GTH21_RS29140 Protein ID WP_000754566.1
Coordinates 31273..31689 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GTH21_RS29145 Protein ID WP_001261276.1
Coordinates 31686..31916 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH21_RS29120 26585..27661 - 1077 WP_000227969.1 IS110 family transposase -
GTH21_RS29125 27943..28122 - 180 Protein_33 IS3 family transposase -
GTH21_RS29130 28203..29711 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
GTH21_RS29135 30384..31069 - 686 Protein_35 IS3-like element ISEc15 family transposase -
GTH21_RS29140 31273..31689 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GTH21_RS29145 31686..31916 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GTH21_RS29150 32490..32840 + 351 WP_000493378.1 hypothetical protein -
GTH21_RS29155 32891..33634 + 744 WP_000129823.1 hypothetical protein -
GTH21_RS29160 33631..34407 + 777 WP_000015958.1 site-specific integrase -
GTH21_RS29165 34465..34722 - 258 WP_000764642.1 hypothetical protein -
GTH21_RS29170 34851..34955 - 105 WP_032409716.1 hypothetical protein -
GTH21_RS29175 35485..36351 + 867 WP_004118283.1 replication initiation protein -
GTH21_RS29180 36528..36797 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ant(3'')-Ia / cmlA1 / aadA2 / sul3 / aac(3)-IVa / aph(4)-Ia - 1..50845 50845


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T146007 WP_000754566.1 NZ_CP047338:c31689-31273 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T146007 NZ_CP062715:c5062571-5062398 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT146007 WP_001261276.1 NZ_CP047338:c31916-31686 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT146007 NZ_CP062715:c5062895-5062599 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References