Detailed information of TA system TA146006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 182324..182849
• Replicon: plasmid p2019036D-mcr8-345kb
• Accession: NZ_CP047337
• Organism: Klebsiella pneumoniae strain 2019036D

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: GTH21_RS27925
• Protein ID: WP_004197633.1
• Coordinates: 182544..182849 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: GTH21_RS27920
• Protein ID: WP_004197642.1
• Coordinates: 182324..182542 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH21_RS27900	177587..178569	+	983	Protein_196	IS5-like element ISKpn26 family transposase	-
GTH21_RS27905	179011..179691	-	681	WP_032445676.1	hypothetical protein	-
GTH21_RS27910	179985..181013	-	1029	WP_032445668.1	Abi family protein	-
GTH21_RS27915	181174..181755	-	582	WP_072310991.1	hypothetical protein	-
GTH21_RS27920	182324..182542	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GTH21_RS27925	182544..182849	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
GTH21_RS27930	183018..183413	+	396	WP_164530166.1	hypothetical protein	-
GTH21_RS27935	183440..183763	+	324	WP_004197641.1	hypothetical protein	-
GTH21_RS27940	183760..184776	+	1017	WP_164530167.1	hypothetical protein	-
GTH21_RS27950	184974..185759	+	786	WP_046664219.1	site-specific integrase	-
GTH21_RS27955	186208..186963	+	756	WP_001568031.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / mcr-8 / dfrA12 / aadA2 / qacE / sul1 / blaDHA-1 / qnrB4 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(D) / floR / blaCTX-M-27	-	1..345655	345655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T146006 WP_004197633.1 NZ_CP047337:182544-182849 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T146006 NZ_CP062715:c4965757-4965407 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT146006 WP_004197642.1 NZ_CP047337:182324-182542 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT146006 NZ_CP062715:c4966002-4965751 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5