Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 78600..79336 Replicon plasmid p2019036D-mcr8-345kb
Accession NZ_CP047337
Organism Klebsiella pneumoniae strain 2019036D

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag GTH21_RS27310 Protein ID WP_003026803.1
Coordinates 78854..79336 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag GTH21_RS27305 Protein ID WP_003026799.1
Coordinates 78600..78866 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH21_RS27260 74662..75024 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
GTH21_RS27265 75074..75424 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
GTH21_RS27270 75782..76051 + 270 WP_004152102.1 hypothetical protein -
GTH21_RS27275 76039..76614 + 576 WP_004152103.1 hypothetical protein -
GTH21_RS27280 76645..77139 + 495 WP_004152104.1 DNA-binding protein -
GTH21_RS27285 77183..77551 + 369 WP_004152105.1 hypothetical protein -
GTH21_RS27290 77585..77788 + 204 WP_004152106.1 hemolysin expression modulator Hha -
GTH21_RS27295 77837..78094 + 258 WP_004152107.1 hypothetical protein -
GTH21_RS27300 78170..78424 + 255 WP_004152108.1 hypothetical protein -
GTH21_RS27305 78600..78866 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
GTH21_RS27310 78854..79336 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
GTH21_RS27315 79537..80940 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
GTH21_RS27320 80969..81601 - 633 WP_001567369.1 hypothetical protein -
GTH21_RS27325 81831..83177 + 1347 WP_020314316.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / mcr-8 / dfrA12 / aadA2 / qacE / sul1 / blaDHA-1 / qnrB4 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(D) / floR / blaCTX-M-27 - 1..345655 345655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T146005 WP_003026803.1 NZ_CP047337:78854-79336 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T146005 NZ_CP062715:4831203-4831544 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT146005 WP_003026799.1 NZ_CP047337:78600-78866 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT146005 NZ_CP062715:c4831208-4831132 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References