Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
Location | 78600..79336 | Replicon | plasmid p2019036D-mcr8-345kb |
Accession | NZ_CP047337 | ||
Organism | Klebsiella pneumoniae strain 2019036D |
Toxin (Protein)
Gene name | tacT | Uniprot ID | L7SZ15 |
Locus tag | GTH21_RS27310 | Protein ID | WP_003026803.1 |
Coordinates | 78854..79336 (+) | Length | 161 a.a. |
Antitoxin (Protein)
Gene name | tacA | Uniprot ID | L7SZ29 |
Locus tag | GTH21_RS27305 | Protein ID | WP_003026799.1 |
Coordinates | 78600..78866 (+) | Length | 89 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
GTH21_RS27260 | 74662..75024 | - | 363 | WP_004152100.1 | arsenite efflux transporter metallochaperone ArsD | - |
GTH21_RS27265 | 75074..75424 | - | 351 | WP_004152101.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
GTH21_RS27270 | 75782..76051 | + | 270 | WP_004152102.1 | hypothetical protein | - |
GTH21_RS27275 | 76039..76614 | + | 576 | WP_004152103.1 | hypothetical protein | - |
GTH21_RS27280 | 76645..77139 | + | 495 | WP_004152104.1 | DNA-binding protein | - |
GTH21_RS27285 | 77183..77551 | + | 369 | WP_004152105.1 | hypothetical protein | - |
GTH21_RS27290 | 77585..77788 | + | 204 | WP_004152106.1 | hemolysin expression modulator Hha | - |
GTH21_RS27295 | 77837..78094 | + | 258 | WP_004152107.1 | hypothetical protein | - |
GTH21_RS27300 | 78170..78424 | + | 255 | WP_004152108.1 | hypothetical protein | - |
GTH21_RS27305 | 78600..78866 | + | 267 | WP_003026799.1 | DUF1778 domain-containing protein | Antitoxin |
GTH21_RS27310 | 78854..79336 | + | 483 | WP_003026803.1 | GNAT family N-acetyltransferase | Toxin |
GTH21_RS27315 | 79537..80940 | + | 1404 | WP_001567368.1 | ISNCY-like element ISKpn21 family transposase | - |
GTH21_RS27320 | 80969..81601 | - | 633 | WP_001567369.1 | hypothetical protein | - |
GTH21_RS27325 | 81831..83177 | + | 1347 | WP_020314316.1 | ISNCY family transposase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | aph(3')-Ia / mph(A) / mcr-8 / dfrA12 / aadA2 / qacE / sul1 / blaDHA-1 / qnrB4 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(D) / floR / blaCTX-M-27 | - | 1..345655 | 345655 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17353.97 Da Isoelectric Point: 9.5822
>T146005 WP_003026803.1 NZ_CP047337:78854-79336 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T146005 NZ_CP062715:4831203-4831544 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10147.59 Da Isoelectric Point: 4.5256
>AT146005 WP_003026799.1 NZ_CP047337:78600-78866 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
Download Length: 267 bp
>AT146005 NZ_CP062715:c4831208-4831132 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0J2GNW6 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A1Q8YL66 |