Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 5322445..5323136 Replicon chromosome
Accession NZ_CP047336
Organism Klebsiella pneumoniae strain 2019036D

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag GTH21_RS26620 Protein ID WP_025987721.1
Coordinates 5322795..5323136 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag GTH21_RS26615 Protein ID WP_019725272.1
Coordinates 5322445..5322771 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH21_RS26590 5317972..5319060 + 1089 WP_019725277.1 hypothetical protein -
GTH21_RS26595 5319143..5320033 + 891 WP_019725276.1 50S ribosome-binding GTPase -
GTH21_RS26600 5320598..5321287 + 690 WP_009309812.1 hypothetical protein -
GTH21_RS26605 5321499..5321945 + 447 WP_025987722.1 antirestriction protein -
GTH21_RS26610 5321955..5322431 + 477 WP_019725273.1 DNA repair protein RadC -
GTH21_RS26615 5322445..5322771 + 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GTH21_RS26620 5322795..5323136 + 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
GTH21_RS26625 5323251..5324084 + 834 WP_025987720.1 DUF4942 domain-containing protein -
GTH21_RS26630 5324171..5324482 + 312 WP_073568733.1 hypothetical protein -
GTH21_RS26635 5324977..5326212 + 1236 WP_124044824.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5299945..5341962 42017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T146004 WP_025987721.1 NZ_CP047336:5322795-5323136 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

>T146004 NZ_CP062715:c4715351-4715037 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT146004 WP_019725272.1 NZ_CP047336:5322445-5322771 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp

>AT146004 NZ_CP062715:c4715587-4715354 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References