Detailed information of TA system TA146002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5075326..5077007
• Replicon: chromosome
• Accession: NZ_CP047336
• Organism: Klebsiella pneumoniae strain 2019036D

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A483YLF8
• Locus tag: GTH21_RS25430
• Protein ID: WP_019724882.1
• Coordinates: 5076090..5077007 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: GTH21_RS25425
• Protein ID: WP_004177809.1
• Coordinates: 5075326..5076084 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH21_RS25410	5071176..5071862	-	687	WP_004151733.1	sel1 repeat family protein	-
GTH21_RS25415	5072092..5074239	-	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
GTH21_RS25420	5074512..5075240	+	729	WP_002885152.1	response regulator transcription factor	-
GTH21_RS25425	5075326..5076084	+	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GTH21_RS25430	5076090..5077007	+	918	WP_019724882.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GTH21_RS25435	5077132..5078139	-	1008	WP_015959270.1	carbohydrate kinase family protein	-
GTH21_RS25440	5078214..5079155	-	942	WP_002885156.1	ABC transporter substrate-binding protein	-
GTH21_RS25445	5079186..5080184	-	999	WP_002885172.1	ribose ABC transporter permease	-
GTH21_RS25450	5080181..5081701	-	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34653.14 Da
• Isoelectric Point: 5.4707

>T146002 WP_019724882.1 NZ_CP047336:5076090-5077007 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CITQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T146002 NZ_CP062715:c4477638-4477240 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT146002 WP_004177809.1 NZ_CP047336:5075326-5076084 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT146002 NZ_CP062715:c4477934-4477641 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A483YLF8

Antitoxin

• AlphaFold DB: A6TH05