Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4758196..4758821 Replicon chromosome
Accession NZ_CP047336
Organism Klebsiella pneumoniae strain 2019036D

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GTH21_RS23935 Protein ID WP_002882817.1
Coordinates 4758438..4758821 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GTH21_RS23930 Protein ID WP_004150355.1
Coordinates 4758196..4758438 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH21_RS23905 4754187..4754786 + 600 WP_004151865.1 glucose-1-phosphatase -
GTH21_RS23910 4754780..4755640 + 861 WP_019725422.1 virulence factor BrkB family protein -
GTH21_RS23915 4755637..4756074 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GTH21_RS23920 4756119..4757060 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GTH21_RS23925 4757074..4757991 - 918 WP_004187175.1 alpha/beta hydrolase -
GTH21_RS23930 4758196..4758438 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GTH21_RS23935 4758438..4758821 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GTH21_RS23940 4758995..4759924 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GTH21_RS23945 4759921..4760556 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GTH21_RS23950 4760553..4761455 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T146001 WP_002882817.1 NZ_CP047336:4758438-4758821 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T146001 NZ_CP062715:4220150-4220368 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT146001 WP_004150355.1 NZ_CP047336:4758196-4758438 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT146001 NZ_CP062715:4219750-4220124 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References