Detailed information of TA system TA145984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 60097..60714
• Replicon: plasmid p116-3
• Accession: NZ_CP047327
• Organism: Enterococcus faecium strain 116

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GST81_RS12330
• Protein ID: WP_231410992.1
• Coordinates: 60097..60249 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GST81_RS12335
• Protein ID: WP_047937794.1
• Coordinates: 60283..60714 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GST81_RS12290 (GST81_12205)	55515..56330	+	816	WP_231410993.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
GST81_RS12295 (GST81_12210)	56333..56818	+	486	WP_002344870.1	single-stranded DNA-binding protein	-
GST81_RS12300 (GST81_12215)	57184..57444	+	261	WP_002293538.1	hypothetical protein	-
GST81_RS12305 (GST81_12220)	57889..58095	+	207	WP_002325537.1	hypothetical protein	-
GST81_RS12310 (GST81_12225)	58095..58346	+	252	WP_212481448.1	hypothetical protein	-
GST81_RS12315 (GST81_12230)	58362..58799	+	438	WP_047927652.1	hypothetical protein	-
GST81_RS12320 (GST81_12235)	58792..59499	+	708	WP_212481450.1	hypothetical protein	-
GST81_RS12325 (GST81_12240)	59793..59972	+	180	WP_002295613.1	hypothetical protein	-
GST81_RS12330 (GST81_12245)	60097..60249	+	153	WP_231410992.1	type II toxin-antitoxin system HicA family toxin	Toxin
GST81_RS12335 (GST81_12250)	60283..60714	+	432	WP_047937794.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GST81_RS12340 (GST81_12255)	60945..61904	-	960	WP_212481452.1	IS30 family transposase	-
GST81_RS13410	62014..62841	+	828	Protein_69	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181744	181744
	-	flank	IS/Tn	-	-	60945..61904	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5727.53 Da
• Isoelectric Point: 10.2749

>T145984 WP_231410992.1 NZ_CP047327:60097-60249 [Enterococcus faecium]
IEKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLEKKILKDAGLK

Nucleotide

• Length: 153 bp

>T145984 NZ_CP062715:408067-408594 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15840.92 Da
• Isoelectric Point: 4.0728

>AT145984 WP_047937794.1 NZ_CP047327:60283-60714 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEELLPEPTPINKISVEDD
SFTTLIRVDVAEYLKETELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT145984 NZ_CP062715:407794-408060 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA