Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 434026..434597 Replicon chromosome
Accession NZ_CP047326
Organism Enterococcus faecium strain 116

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag GST81_RS02075 Protein ID WP_002286801.1
Coordinates 434026..434367 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag GST81_RS02080 Protein ID WP_002323011.1
Coordinates 434367..434597 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GST81_RS02050 (GST81_02045) 429236..430570 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
GST81_RS02055 (GST81_02050) 430778..431608 + 831 WP_002286789.1 manganese catalase family protein -
GST81_RS02060 (GST81_02055) 431852..432101 - 250 Protein_413 transposase -
GST81_RS02065 (GST81_02060) 432292..432486 - 195 WP_002295146.1 hypothetical protein -
GST81_RS02070 (GST81_02065) 432991..433176 - 186 WP_002304207.1 hypothetical protein -
GST81_RS02075 (GST81_02070) 434026..434367 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GST81_RS02080 (GST81_02075) 434367..434597 - 231 WP_002323011.1 hypothetical protein Antitoxin
GST81_RS02085 (GST81_02080) 434884..435222 + 339 WP_002286804.1 hypothetical protein -
GST81_RS02090 (GST81_02085) 435427..436002 - 576 WP_002293673.1 SOS response-associated peptidase family protein -
GST81_RS02095 (GST81_02090) 436488..437069 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
GST81_RS02100 (GST81_02095) 437252..437878 - 627 WP_002286816.1 cysteine hydrolase -
GST81_RS02105 (GST81_02100) 437900..439231 - 1332 WP_192192681.1 FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T145982 WP_002286801.1 NZ_CP047326:c434367-434026 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T145982 NZ_CP062715:283377-283484 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT145982 WP_002323011.1 NZ_CP047326:c434597-434367 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT145982 NZ_CP062715:c283329-283263 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References