145974

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2846069..2846985 Replicon chromosome
Accession NZ_CP047325
Organism Bacillus subtilis strain GOT9

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag GSY53_RS14770 Protein ID WP_003244695.1
Coordinates 2846239..2846985 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag GSY53_RS14765 Protein ID WP_003232646.1
Coordinates 2846069..2846239 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSY53_RS14730 2842932..2843261 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
GSY53_RS14735 2843258..2843422 + 165 WP_003232658.1 XkdX family protein -
GSY53_RS14740 2843466..2844305 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
GSY53_RS14745 2844358..2844627 + 270 WP_003232655.1 hemolysin XhlA family protein -
GSY53_RS14750 2844640..2844903 + 264 WP_003232653.1 phage holin -
GSY53_RS14755 2844916..2845809 + 894 WP_029317679.1 N-acetylmuramoyl-L-alanine amidase -
GSY53_RS14760 2845846..2845983 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
GSY53_RS14765 2846069..2846239 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
GSY53_RS14770 2846239..2846985 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
GSY53_RS14775 2847095..2848096 - 1002 WP_003232642.1 inorganic phosphate transporter -
GSY53_RS14780 2848109..2848726 - 618 WP_003218470.1 DUF47 domain-containing protein -
GSY53_RS14785 2849002..2850318 - 1317 WP_003232640.1 serine/threonine exchanger -
GSY53_RS14790 2850707..2851657 + 951 WP_003232637.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T145974 WP_003244695.1 NZ_CP047325:c2846985-2846239 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T145974 NZ_CP062713:4480261-4480521 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT145974 WP_003232646.1 NZ_CP047325:c2846239-2846069 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT145974 NZ_CP062713:4479982-4480242 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References