Detailed information of TA system TA145973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2032056..2032692
• Replicon: chromosome
• Accession: NZ_CP047325
• Organism: Bacillus subtilis strain GOT9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GSY53_RS10435
• Protein ID: WP_003156187.1
• Coordinates: 2032342..2032692 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: GSY53_RS10430
• Protein ID: WP_003225183.1
• Coordinates: 2032056..2032337 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSY53_RS10410	2028415..2029014	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
GSY53_RS10415	2029109..2029474	+	366	WP_120363269.1	holo-ACP synthase	-
GSY53_RS10420	2029640..2030656	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
GSY53_RS10425	2030771..2031940	+	1170	WP_003234284.1	alanine racemase	-
GSY53_RS10430	2032056..2032337	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GSY53_RS10435	2032342..2032692	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GSY53_RS10440	2032807..2033631	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
GSY53_RS10445	2033636..2034001	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
GSY53_RS10450	2034005..2034406	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
GSY53_RS10455	2034418..2035425	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
GSY53_RS10460	2035487..2035816	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
GSY53_RS10465	2035813..2036295	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
GSY53_RS10470	2036261..2037049	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
GSY53_RS10475	2037049..2037648	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T145973 WP_003156187.1 NZ_CP047325:2032342-2032692 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T145973 NZ_CP062713:c4474262-4473864 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT145973 WP_003225183.1 NZ_CP047325:2032056-2032337 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT145973 NZ_CP062713:c4474558-4474265 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32