Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2032056..2032692 Replicon chromosome
Accession NZ_CP047325
Organism Bacillus subtilis strain GOT9

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GSY53_RS10435 Protein ID WP_003156187.1
Coordinates 2032342..2032692 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag GSY53_RS10430 Protein ID WP_003225183.1
Coordinates 2032056..2032337 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSY53_RS10410 2028415..2029014 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
GSY53_RS10415 2029109..2029474 + 366 WP_120363269.1 holo-ACP synthase -
GSY53_RS10420 2029640..2030656 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
GSY53_RS10425 2030771..2031940 + 1170 WP_003234284.1 alanine racemase -
GSY53_RS10430 2032056..2032337 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GSY53_RS10435 2032342..2032692 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GSY53_RS10440 2032807..2033631 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
GSY53_RS10445 2033636..2034001 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
GSY53_RS10450 2034005..2034406 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
GSY53_RS10455 2034418..2035425 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
GSY53_RS10460 2035487..2035816 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
GSY53_RS10465 2035813..2036295 + 483 WP_003234299.1 anti-sigma B factor RsbW -
GSY53_RS10470 2036261..2037049 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
GSY53_RS10475 2037049..2037648 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T145973 WP_003156187.1 NZ_CP047325:2032342-2032692 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T145973 NZ_CP062713:c4474262-4473864 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT145973 WP_003225183.1 NZ_CP047325:2032056-2032337 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT145973 NZ_CP062713:c4474558-4474265 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References