Detailed information of TA system TA145972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 20095..20620
• Replicon: plasmid pRM13672
• Accession: NZ_CP047324
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain RM13672

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: GTH51_RS23700
• Protein ID: WP_001159863.1
• Coordinates: 20315..20620 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: GTH51_RS23695
• Protein ID: WP_000813641.1
• Coordinates: 20095..20313 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH51_RS23670	15382..15763	+	382	Protein_20	YgiW/YdeI family stress tolerance OB fold protein	-
GTH51_RS23675	15932..16376	+	445	Protein_21	site-specific integrase	-
GTH51_RS23680	16635..17624	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
GTH51_RS23685	18118..18414	-	297	WP_001687482.1	hypothetical protein	-
GTH51_RS23690	18898..19419	-	522	WP_010999942.1	hypothetical protein	-
GTH51_RS23695	20095..20313	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GTH51_RS23700	20315..20620	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
GTH51_RS23705	20622..20912	+	291	WP_001266176.1	hypothetical protein	-
GTH51_RS23710	20909..21430	+	522	WP_000198608.1	hypothetical protein	-
GTH51_RS23715	21465..22247	+	783	WP_000082169.1	site-specific integrase	-
GTH51_RS23720	22256..22969	+	714	Protein_30	EAL domain-containing protein	-
GTH51_RS23725	22973..23482	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
GTH51_RS23730	23476..23961	+	486	WP_000905606.1	membrane protein	-
GTH51_RS23735	24238..24525	-	288	WP_071530243.1	hypothetical protein	-
GTH51_RS23740	24681..25241	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93832	93832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T145972 WP_001159863.1 NZ_CP047324:20315-20620 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T145972 NZ_CP062713:4216778-4216996 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT145972 WP_000813641.1 NZ_CP047324:20095-20313 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT145972 NZ_CP062713:4216378-4216752 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6